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. 2004 Aug 30;166(5):685–696. doi: 10.1083/jcb.200406181

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Copyright © 2004, The Rockefeller University Press

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Figure 3. — Tm5NM-34kd is expressed in slow fibers, whereas the γ9d isoforms are expressed in all major fiber types. Consecutive transverse sections (7 μm) through the soleus muscle shows that fibers that were positive for the CG3 antibody (A and B, asterisks) were slow fibers (stained with the slow myosin heavy chain (MHC) antibody in B. Sections were stained with the primary antibodies followed by peroxidase-conjugated goat/anti–mouse secondary antibodies. In contrast to CG3, γ9d stained all fibers in soleus (C) and EDL (E) muscles. Note that between them the soleus and EDL contain all adult skeletal muscle fiber types (types I, IIA, IIX, and IIB). Negative (−ve) control sections (D and F) with the secondary antibody (donkey anti–sheep Alexa 488) confirms the specificity of the staining with γ9d. Bars, 40 μm.