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. 2004 Dec 6;167(5):945–952. doi: 10.1083/jcb.200404108

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Copyright © 2004, The Rockefeller University Press

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Figure 5. — The Shank3 proline-rich domain is crucial for Ret-induced tubule formation. MDCK cell lines expressing fusion proteins of the full-length Ret9 receptor with either the NH₂ terminus of Shank3 (Ret–Shank3 NT; a–c) or the proline-rich domain of Shank3 (Ret9–Shank3–Pro1; d–f) were examined for tubulogenesis in three-dimensional collagen matrices. After stimulation with GDNF–sGFRα1 or HGF/SF, cell cultures were fixed and photographed. Bar in f: 200 μm (applies to a–f). (g) Expression of the indicated cDNA constructs shown by RT-PCR.