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. 2004 Dec 6;167(5):875–887. doi: 10.1083/jcb.200408001

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Copyright © 2004, The Rockefeller University Press

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Figure 1. — Sec1p and Sec4p overexpression stimulate growth and secretion of a sec3Δ mutant strain. (A) Wild-type (left) and sec3Δ mutant strains (right) carrying the indicated multi-copy (2μ) plasmids were grown on SC plates for 3 d at 25°C. After the loss of the 2μSEC1 plasmid on the 5FOA plate (bottom left), the previously suppressed sec3Δ strain is slow growing comparable to the strain with the control empty plasmid (top left). (B) Invertase secretion was measured in wild-type, sec3Δ, and sec3Δ strains suppressed by either 2μSEC1 or 2μSEC4. To derepress invertase, the cells were shifted to low glucose (0.1%) containing SC medium at 25°C. After 90 min incubation in this medium, invertase activity was measured. The graph shows the amount of intracellular invertase as a percentage of the total newly synthesized invertase. For each strain the average and SD from four independent experiments are shown.