Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2004 Dec 6;167(5):875–887. doi: 10.1083/jcb.200408001

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2004, The Rockefeller University Press

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).

PMC Copyright notice

Figure 7. — Co-isolation of Sec1p and exocyst subunits with myc epitope tagged Sec8p. The exocyst was isolated from lysates of wild-type (B and controls in A, C, and D), sec3Δ (A), sec5Δ (C), and exo70Δ (D) mutant strains as described in Materials and methods. Except for the control strains (no tag), all strains express Sec8p with a carboxy-terminal 13myc epitope. For the exocyst isolation, myc epitope–tagged Sec8p was immunoprecipitated with a monoclonal myc antibody. Co-immunoprecipitation of Sec1p, Sec6p, Sec10p, Sec15p, and Ssop was detected by Western blotting. Mutant genotypes (wt, sec3Δ, sec5Δ, or exo70Δ) and the presence of multi-copy plasmids (2μSEC1 or 2μSEC4) are indicated on the top of each lane. 30% of the immunoprecipitates (IP, right panels) is compared with 1% of the lysates (left panels). Major nonspecific bands in the lysates are marked with an asterisk. Two exposures of the Sec1p Western blot are shown in A.