Table II. ER tubule analysis.

Strain description	Tubule number	Tubule dynamic	Tubule orientation
wt	2	+	+
wt 2μSEC1	2	+	+
wt 2μSEC4	2	+	+
sec3Δ	3	+	+
sec3Δ 2μSEC1	3	+	+
sec3Δ 2μSEC4	2	+	+
sec5Δ 2μSEC1	4	+	+
sec5Δ 2μSEC4	2	+	+
exo70Δ 2μSEC1	3	+	+
exo70Δ 2μSEC4	3	+	+

Yeast strains were analyzed to determine the average number of ER tubules per cell, the tubule dynamic of each, and whether or not at least one tubule is oriented towards the bud. At least eight movies were analyzed for each strain (see supplemental materials, available at http://www.jcb.org/cgi/content/full/jcb.200408001/DC1).