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. 2004 Dec 6;167(5):863–874. doi: 10.1083/jcb.200407022

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Copyright © 2004, The Rockefeller University Press

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Figure 1. — Localization of DGDG in mitochondria of A. thalia na cells deprived of P_i for 3 d. Cells (A, control; B–D, P_i-deprived) were processed for indirect immunofluorescence labeling using anti-DGDG with secondary antibodies coupled to BODIPY and either anti-BCCP1, for chloroplast detection (A and B), or anti-HPPK (C), for mitochondria detection, with secondary antibodies coupled to Alexa 594. In D, mitochondria were visualized by staining with Mitotracker orange CMTMRos. Cells were observed by confocal microscopy. Bars: A–C, 8 μm; D, 20 μm.