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. 2004 Nov 8;167(3):555–562. doi: 10.1083/jcb.200404142

Figure 3.

Figure 3.

pS368 levels rise relative to total Cx43 in response to scratch wounding in a manner dependent on PKC activity. (A) Time course after wounding of the ratio of pS368 to total Cx43, normalized to the zero time level. (B) pS368/Cx43 levels 24 h after wounding in the presence and absence of kinase inhibitors, normalized to the control level. Control was not scrape wounded but was harvested at the same time as the wounded samples. BIM, bisindolylmaleimide (200 nM); Ro-31, Ro-31-8220 (100 nM); and Gö6976 (100 nM). (A and B) Error bars represent standard error of the mean. (C) The COOH-terminal region of Cx43 (residues 246–382) was phosphorylated with PKCɛ and immunoblotted as described in Materials and methods. Phosphorylation and total Cx43 were detected with antibodies to pS368 and the COOH-terminal region of Cx43. (D) Representative immunoblot of the PKC inhibitor data summarized in B. Note that the migration of the pS368 signal overlays with the fastest migrating species of Cx43 and there is a higher level of total Cx43 in the control because cells were not lost during scratch wounding.