Figure 6.

Abp1p-GFP and Sac6p-HcRed assemble at the same punctate structures in living yeast. Wild-type haploid cells expressing Abp1p-GFP and Sac6p-HcRed from the chromosomal loci were grown to mid-log phase in lactate medium and imaged in a single cortical focal plane using simultaneous two-color imaging as for Fig. 1. Images shown are still frames from a time-lapse series showing Abp1p-GFP–labeled actin patches in the top row, Sac6p-HcRed–labeled actin patches in the middle row, and a merged image showing Abp1p-GFP in green and Sac6p-HcRed in red in the bottom row. Arrowheads indicate the point of emergence of fluorescent signal in the top and middle rows. The outline of the cell is shown in the top panel at t = 0 s. Bar, 2 μm.