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. 2004 Nov 8;167(3):479–492. doi: 10.1083/jcb.200403093

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Copyright © 2004, The Rockefeller University Press

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Figure 3. — FAIM increases NGF-induced neurite outgrowth in mouse SCG neurons. Ballistic transfections of cultured neurons with an eYFP expression plasmid together with the Neo control vector or FAIM-S plasmids. After 24-h incubation with NGF (10 ng/ml), eYFP-labeled SCG were visualized and digitally acquired. (A) Representative drawings of the neurons corresponding to the 25, 50, 75, and 100 percentiles of the neurite length are shown. (B) Representative micrographs showing the FAIM-increased neuritic outgrowth. Bars, 50 μm. (C) Quantification of neurite length (top) and branching points (bottom). Significant differences in neurite length between Neo and FAIM are indicated (*, P < 0.05 and **, P < 0.001, respectively; t test).