Figure 6.

NF-κB activation is modulated by FAIM. (A) PC12 cells were stably transfected with an empty vector (Neo), with the FAIM-S expression plasmid, or with the FAIM RNAi plasmid. Cells were then transiently transfected with an NF-κB–dependent reporter vector (LTR KB) or with the equivalent control vector lacking NF-κB binding sites (LTR deltaKB). After 24 h, the cells were treated with NGF (100 ng/ml) for the indicated times and luciferase activity was measured in the cell lysates using a Luciferase Assay System. Luciferase values were normalized to protein concentration (RLU/μg of protein). (B) Immunocytochemistry was performed to detect the p65 subunit of NF-κB in similar cells as described in A. Representative low and high magnification photomicrographs are shown (arrowheads indicate the selected cells illustrated in the insets). Bar, 25 μm. (C) Graph showing the percentage of nuclear p65 staining in the different experimental conditions. The mean ± SEM of three independent experiments are shown.