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. 2004 Nov 8;167(3):479–492. doi: 10.1083/jcb.200403093

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Copyright © 2004, The Rockefeller University Press

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Figure 8. — FAIM-induced increase in neurite outgrowth is eliminated in cortical neurons from p65 ⁻ ^/ ⁻ mouse embryos. Cultured cortical neurons from p65^+/+ (WT) or p65^−/− (KO) mice were transfected with eYFP alone or with eYFP plus FAIM-S. After 24 h, labeled cortical neurons were visualized, digitally acquired, and measured. (A) Drawings of neurons that represent the 25, 50, 75, and 100 percentiles of neurite length measurements. (B) Micrograph showing representative cells of p65^+/+ (WT) or p65^−/− genotypes (KO) transfected with the indicated plasmids. (C) Quantification of the neurite outgrowth performed by tracing the neuritic arbors and measuring the neuritic length (top) and branching points (bottom). Bars, 50 μm. *, P < 0.001; t test.