Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2004 Nov 8;167(3):479–492. doi: 10.1083/jcb.200403093

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2004, The Rockefeller University Press

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).

PMC Copyright notice

Figure 9. — FAIM interacts with TrkA and p75NTR receptors in an NGF-dependent manner. Wild-type PC12 cells were electroporated with FLAG-tagged FAIM-S and either TrkA-HA (A) or p75^NTR-HA (B). After 48 h, the cells were stimulated (+) or not (−) with 100 ng/ml NGF for 15 min. TrkA-HA (A) or FAIM-FLAG (B) were immunoprecipitated from 1 mg of cell lysate followed by Western blotting with either FLAG or HA antibody. An IP with myc antibody was used as a negative IP control in B. A fraction of the lysate was blotted with FLAG, HA, or phospho-ERKs antibody (top). (C) Wild-type PC12 cells were electroporated with FLAG-FAIM-S and were stimulated (+) or not (−) with 100 ng/ml NGF for 15 min. FLAG-FAIM-S was immunoprecipitated from 1 mg of cell lysate with the FLAG antibody, and Western blotting was used to detect endogenous Trk with anti-Trk antibody 203 in the precipitates. A fraction of the lysate was blotted with FLAG or phospho-ERKs antibody (top).