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. 2004 Nov 8;167(3):425–432. doi: 10.1083/jcb.200406068

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Copyright © 2004, The Rockefeller University Press

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Figure 3. — Targeted disruption of PbIMC1a. (A) Diagram of the recombination strategy. Indicated are sequences specific for PbIMC1a (dark gray); PbDHFR (light gray); TgDHFR/TS (white); positions of SphI restriction sites (s); and probes used in Southern blot. (B) Southern analysis of SphI-digested genomic DNA (gDNA) from WT and PbIMC1a-KO parasite clones 104 and 204. (C) RT-PCR analysis of blood stages total RNA for mRNA of PbIMC1a and the reference gene tubulin-1.