Table I. Effects of PbIMC1a knockout on P. berghei parasite development in A. stephensi mosquitoes.
| Mean no. of sporozoites per mosquitob
|
||||||
|---|---|---|---|---|---|---|
| Experiment | Parasite [clone] | Mean ± SEM no. of oocysts per mosquitoa | Percent infection | Midgut | Hemolymph | Salivary glands |
| I | WT | 268 ± 34 (30) | 100 | 10,190 (10) | N/A | 6,880 (10) |
| PbIMC1a-KO [104] | 225 ± 30 (29) | 100 | 12,420 (10) | N/A | 0 (10) | |
| PbIMC1a-KO [204] | 270 ± 33 (30) | 100 | 24,000 (10) | N/A | 0 (10) | |
| II | WT | 380 ± 53 (10) | 100 | 26,630 (10) | N/A | 1,940 (10) |
| PbIMC1a-KO [104] | 302 ± 69 (10) | 100 | 14,000 (10) | N/A | 0 (10) | |
| III | WT | 118 ± 19 (20) | 100 | 31,000 (20) | 4,800 (20) | 69,660 (20) |
| PbIMC1a-KO [104] | 127 ± 18 (20) | 100 | 36,330 (20) | 1,530 (20) | 0 (19) | |
Number of mosquitoes per sample indicated in parentheses. N/A, not assessed.
a
Assessed at 9–11 d after infection. bAssessed at 22–24 d after infection.