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. 2004 Nov 8;167(3):493–504. doi: 10.1083/jcb.200405039

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Copyright © 2004, The Rockefeller University Press

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Figure 1. — Expression of neogenin, netrin-3, CDO, and muscle-specific proteins in C2C12, F3, and 10T1/2 cells. (A) Western blot analysis of C2C12 cell differentiation. Cultures were brought to ∼90% confluence (day 0) and shifted into DM for the indicated period of time and harvested. Western blots were probed with antibodies against the indicated proteins. Immunoblotting for myogenin and MHC indicates the progression of cell differentiation; immunoblotting with a pan-cadherin antibody serves as a loading control (Kang et al., 2003). (B) Western blot analysis of F3 myoblasts cultured in GM (G) or DM (D) for 2 d. Blots were probed with antibodies against the indicated proteins. (C) Western blot analysis of 10T1/2 cell derivatives. 10T1/2 cells stably transfected with a control expression vector (−) or with a MyoD expression vector (+) were cultured in DM for 24 h. Blots were probed with antibodies against the indicated proteins. (D) Northern blot analysis of netrin-3 mRNA expression during C2C12 cell differentiation. The ethidium bromide-stained gel is shown as a loading control.