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. 2004 Nov 8;167(3):493–504. doi: 10.1083/jcb.200405039

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Copyright © 2004, The Rockefeller University Press

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Figure 4. — Netrin-2 promotes myotube formation. (A) Photomicrographs of C2C12 cells were treated with 5% FBS plus or minus recombinant chicken netrin-2 for 24 h, and fixed and stained with an antibody to MHC. Bars: (top) 0.5 mm; (bottom) 0.2 mm. (B) Quantification of myotube formation. Values represent means of triplicate determinations ± 1 SD. (C) Western blot analysis of muscle-specific proteins by C2C12 cells treated with 5% FBS plus or minus recombinant chicken netrin-2 for 24 h. Cell lysates were probed with the indicated antibodies.