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| Strains mated | No. of fused mitochondria /total scored |
Percent fusion observed |
|---|---|---|
|
GEM1 DNM1 UGO1 × GEM1 DNM1 UGO1 |
157/160 | 98.1 |
|
gem1Δ DNM1 UGO1 × gem1Δ DNM1 UGO1 |
177/206 | 85.9 |
|
gem1Δ dnm1Δ UGO1 × gem1Δ dnm1Δ UGO1 |
131/162 | 80.9 |
|
GEM1 dnm1Δ ugo1Δ × GEM1 dnm1Δ ugo1Δ |
0/157 | 0.0 |
Large-budded zygotes containing both mito-RFP and mito-GFP were scored at 2.5–4 h after mating on YPD.
