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. 2004 Oct 11;167(1):51–63. doi: 10.1083/jcb.200404120

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Copyright © 2004, The Rockefeller University Press

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Figure 4. — NAPs surround transcriptionally active NORs during telophase. During telophase, endogenous SF2/ASF localizes in NAPs (a, arrow) surrounding foci of fibrillarin (b, arrow) in HeLa cells. Endogenous SF2/ASF (d, arrow) is localized in NAPs surrounding foci of fibrillarin (e, arrow) in the nontransformed cell line IMR90. Endogenous SF2/ASF (h, arrow) is localized in NAPs surrounding foci of fibrillarin (i, arrow) in U2OS cells. SF2/ASF is also in MIGs at this stage (a–k, arrowheads). Endogenous SF2/ASF (l, arrow) surrounds foci of the RNA pol I transcription factor upstream binding factor (m, arrow) in HeLa cells. RNA-FISH for ribosomal RNA (q, arrows) showed that SF2/ASF NAPs (p, arrows) surrounded transcriptionally active NORs. RNA-FISH using oligo dT probes demonstrated that polyadenylated RNA (u, arrows) was absent from SF2/ASF NAPs (t, arrows). DNA was stained with DAPI (g, k, o, s, and w). NAP position is indicated by arrows (a–w). Bars, 5 μm.