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. 2004 Oct 25;167(2):327–337. doi: 10.1083/jcb.200403091

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Copyright © 2004, The Rockefeller University Press

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Figure 7. — Chemotactic migration of EL4.G8 cells toward SDF-1 and augmentation of the activity in T567D ezrin transfectants. Chemotactic activity was determined by constant-period counting using a flow cytometer and is shown as mean ± SD (A–C). (A) EL4.G8 cells exhibit typical chemotaxis toward SDF-1 (10 ng/ml), and this chemotaxis is markedly inhibited by pretreatment of the cells with pertussis toxin (PTx), staurosporine, or cytochalasin D. (B) EL4.G8 cell chemotaxis is dependent on ROCK activity. Cells were pretreated with Y-27632 and the chemotaxis assay was performed in medium containing the drug (in) or in medium only (wash out [w/o]). (C) T567D ezrin enhances chemotaxis. Transfectants preincubated with or without PTx were examined by the chemotaxis assay. Numbers represent the fold increase of chemotaxis toward SDF-1 (specific activity) compared with that of cells incubated in medium alone. A result typical of at least three experiments is shown.