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. 2004 Oct 25;167(2):281–292. doi: 10.1083/jcb.200407088

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Copyright © 2004, The Rockefeller University Press

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Figure 7. — The COOH-terminal region of human GMAP-210 mediates targeting to the Golgi rather than centrosomes. (A) Confocal micrographs of COS cells expressing the NH₂-terminal 372 amino acids of GMAP-210 COOH-terminally tagged with GFP, or the COOH-terminal 223 amino acids of GMAP-210 NH₂-terminally tagged with GFP, or full-length GMAP-210 with an NH₂-terminal myc tag. Cells were also labeled with antibodies against the endogenous Golgi protein GM130 or γ-tubulin. At very high levels of GMAP-210, normal Golgi morphology (arrow) is lost and the Golgi becomes fragmented as described previously (Pernet-Gallay et al., 2002), and yet γ-tubulin is still only on the centrosome (arrowheads indicate the centrosomes shown in merged inset). (B and C) Illustrative confocal images of COS cells expressing portions of GMAP-210 fused to GFP and costained with GM130 as in A, along with a summary of the results from all GMAP-210 portions examined.