Table II.
Spindle behavior in bud6 , dyn1 , and bud6dyn1 mutants
| Strain | % correctly aligned spindles at onset of anaphasea |
% late anaphase spindles exhibiting correct polarityb |
|---|---|---|
| wild type | 100 (n = 28) | 100 (n = 200) |
| bud6Δ | 87 (n = 15) | 92 (n = 300) |
| dyn1Δ | 94c (n = 35) | 90 (n = 210) |
| bud6Δ dyn1Δ | 72 (n = 18) | 60 (n = 302) |
a
Alignment of the spindle at anaphase onset was scored as described in Table I. Results are expressed as percentage of total anaphase cells recorded (n).
b
Polarity in late anaphase spindles was considered correct if a single pole was still connected to the bud cortex, irrespective of spindle alignment. Results are expressed as percentage of total anaphase cells counted (n) in asynchronous populations of the indicated strains.
c
In 9% of anaphase cells recorded, the spindle became overtly misaligned during anaphase, after initial elongation along the mother-bud axis (Fig. 8 C).