Table III.
Effect of tub2 C354S on spindle alignment in kar9 Δ cells
| Strain | % spindle alignmenta
at anaphase onset in time-lapse series |
% correct preanaphaseb
spindle orientation in asynchronous cells |
|---|---|---|
| wild type | 100 (n = 28) | 98 ± 2 |
| kar9Δ | 60 (n = 25) | 63 ± 4 |
| kar9Δ tub2 C354S | 86 (n = 22) | 90 ± 3 |
| kar9Δ tub C354S bud6Δ | 25 (n = 40) | 29 ± 4 |
| kar9Δ bud6Δ | 33 (n = 39) | 32 ± 3 |
| bud6Δ tub2 C354S | 80 (n = 20) | 72 ± 3 |
| bud6Δ | 87 (n = 15) | 79 ± 4 |
a
Scoring was carried out in time-lapse series spanning early anaphase as indicated in Table I. n = total anaphase cells recorded.
b
Spindle orientation was scored in 500 preanaphase cells in asynchronous cultures of the indicated strains expressing GFP-Tub1. Results were expressed as mean ± SD for triplicate experiments. Alignment of the preanaphase spindle was considered correct if an imaginary line drawn through the long axis of the spindle transversed the mother-bud neck (Theesfeld et al., 1999).