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. 1998 Sep 29;95(20):11834–11839. doi: 10.1073/pnas.95.20.11834

Figure 2.

Figure 2

The expression of introduced 2B4TCR α gene in thymus. Thymocytes from KI mice were stained with phycoerythrin-anti-CD4 antibody, and fluorescein isothiocyanate-anti-CD8 antibody, and biotin-A2B4 antibody or biotin-anti-CD3 antibody as described in Materials and Methods. (A) CD4 and CD8 expression pattern of total thymocytes and A2B4 positive thymocytes. (B) Four different subpopulations (CD4+CD8−, CD4+CD8+, CD4−CD8−, and CD4−CD8+) of thymocytes of KI mouse were gated and analyzed for the expression of KI TCR gene. (C) The comparison of CD3 and KI TCR α chain expression in CD4−CD8− DN thymocyte population.