Figure 3.

Deletion of the KI TCR in the thymus. (A) Thymocytes from KI/TCR α⁻ mice were stained as described in Fig. 2. (B) PCR analysis was performed on DNA extracted from 13 hybridoma clones from 2B4 KI mice to test the presence of the KI TCR gene. One hybridoma was stained positive with A2B4 antibody, and others were negative. Primers used were the same as the ones for the screening of homologous recombination. PCR analysis was performed on the same DNA by using actin primers.