Figure 4.

Expression of introduced TCR α gene in the thymus of KI TCR/2B4 TCR β mice. Thymocytes from KI and KI/2B4 TCR β mice (H-2 kxb) were stained as described in Fig. 2. (A) 2B4⁺ thymocytes from KI TCR and KI TCR/2B4 TCR β mice were gated to illustrate their CD4 and CD8 expression patterns. (B) Four different subpopulations (CD4⁺CD8⁻, CD4⁺CD8⁺, CD4⁻CD8⁻, and CD4⁻CD8⁺) cells from KI TCR/2B4 TCR β thymus were gated and analyzed for the expression of the KI TCR gene in these populations.