Figure 6.

Effect of negative selection on thymocytes of 2B4 αβ TCR transgenic and KI TCR/2B4 TCR β mice. (A) Thymocytes from 2B4 αβ TCR transgenic mice and KI TCR/2B4 TCR β with or without transgenic Cyt c gene were stained as described in Fig. 2. Thymocyte subsets distribution in total thymocyte and A2B4-positive thymocytes are shown. All mice have MHC background kxb, and the number of total thymocytes is shown in parenthesis. (B) Thymocytes from KI TCR/2B4 TCR β mouse with or without Cyt c transgene were stained with anti-CD4, anti-CD8, and A2B4. Thymocytes subsets distribution is shown at left. CD4 CD8 DN and CD4 SP thymocytes were gated and analyzed for the expression of 2B4 TCR α chain.