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. 2004 Dec 20;167(6):1183–1194. doi: 10.1083/jcb.200403043

Table II. Chronological order of mechanisms required during the development of adhesion in paired Ecad cells.

Time (min)
0–0.5
0.5–4
4–30
30+
Molecular manipulation
or drug treatment		 Effects First phase
of adhesion		 Second phase
of adhesion		 Third phase
of adhesion
Deletion of E-cadherin cytoplasmic domain No interaction with  cytoplasmic partners     −     +         +     +
Without calcium anti-cadherin antibody Nonfunctional  cadherins     +     +         +     +
E-cadherin–α-catenin chimera Direct interaction
  with the
  actin cytoskeleton		     −     −         −     −
Latrunculin Inhibition of actin  polymerization     −     +         +     +
Jasplakinolide Aggregation/  polymerization of actin
Brefeldin A Inhibition of vesicular  transport     −     −         − nd
Actin network organizationand membrane dynamics
Cdc42DN/RacDN Inhibition of filopodia/  lamellipodia and  membrane ruffles     −     −         + nd
Cdc42DA/RacDA Stimulation of filopodia/  lamellipodia and  membrane ruffles     +     +         +     +
RhoDN/RhoDA Inhibition/induction of  stress fibers; cell  contractility     −     −         −     −
E-cadherin E-cadherin E-cadherin E-cadherin
Dependency Actin
polymerization		 Actin
polymerization
Actin remodelling
through Rac/Cdc42
activation		 Actin dynamics
Membrane
dynamics		 Membrane
dynamics		 Membrane
dynamics

+, sensitive.

−, insensitive.

nd, not done.