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. 2004 Dec 20;167(6):1113–1122. doi: 10.1083/jcb.200408079

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Figure 6. — Frequency and expansion capabilities of CFU-F. (top) Frequency of CFU-F at the time of explantation (t₀). Results shown as number of colonies per 10⁵ nucleated bone marrow cells (mean of triplicate determinations per mouse). The tg marrow is reduced in CFU-F, relative to the wt marrow (analysis of variance [ANOVA], Scheffe's F test 309.16* [significant at 95%], P < 0.0001). (middle) Frequency of CFU-F at the end of ex vivo expansion (day 17, t_N). Results shown as number of colonies per 10³ stromal cells (mean of triplicate determinations per two cell strains per genotype). Strains of tg mice are enriched in total CFU-F, relative to strains of wt mice (ANOVA, Scheffe's F test 94.03* [significant at 95%], P < 0.0002). (bottom) Fold increase in CFU-F over the culture period was calculated as the ratio of the total number of CFU-Fs at the end of the culture period to the total number of CFU-Fs in the marrow explants. Results based on triplicate determinations in duplicate experiments (ANOVA, Scheffe's F test 124.52* [significant at 95%], P = 0.02). (top, middle, and bottom) Error bars indicate SD of the mean.