Figure 2.

Granzyme B uptake into K44A mutant dynamin–overexpressing HeLa cells is not inhibited by a vast molar excess of M6P. Cytofluorographic analysis of HeLa cells overexpressing K44A mutant dynamin (−tet, bottom) or expressing only wild-type dynamin (+tet, top) after incubation with FITC–granzyme B (50 nM) at 37°C for 20 min or in the absence of FITC–granzyme B (filled traces). Cells were preincubated either with 5 mM M6P (red) or G6P (blue) or without competing monosaccharide (green) for 15 min at 37°C.