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. 2003 Jan 20;160(2):201–212. doi: 10.1083/jcb.200207045

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Copyright © 2003, The Rockefeller University Press

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Figure 3. — Golgin-84 is not part of the Golgi matrix. (a) Rat liver Golgi extracts were immunoprecipitated under native conditions with antibodies to either golgin-84, GM130, or p115, or with a control IgG. The immunoprecipitated (IP) and unbound fractions were analyzed by Western blotting with antibodies to golgin-84, GM130, p115, GRASP65, and mannosidase I as indicated. (b) NRK cells were incubated with 5 μg/ml BFA for 1 h and then fixed and double labeled with antibodies to golgin-84 and GM130 or the ER marker calnexin. Bar, 10 μm.

Figure 3. — Golgin-84 is not part of the Golgi matrix. (a) Rat liver Golgi extracts were immunoprecipitated under native conditions with antibodies to either golgin-84, GM130, or p115, or with a control IgG. The immunoprecipitated (IP) and unbound fractions were analyzed by Western blotting with antibodies to golgin-84, GM130, p115, GRASP65, and mannosidase I as indicated. (b) NRK cells were incubated with 5 μg/ml BFA for 1 h and then fixed and double labeled with antibodies to golgin-84 and GM130 or the ER marker calnexin. Bar, 10 μm.