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. 2003 Jan 20;160(2):201–212. doi: 10.1083/jcb.200207045

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Copyright © 2003, The Rockefeller University Press

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Figure 8. — Depletion of golgin-84 partially inhibits transport of VSV-G from the ER to the cell surface. Control or golgin-84 RNAi–treated HeLa cells were transfected with a plasmid encoding GFP-tagged ts045G VSV-G protein. Cells were incubated at 39.5°C to arrest ts045G VSV-G in the ER, and then chased at 31°C for various times to allow transport before fixation and labeling for cell surface VSV-G. (a) An example of cells shifted to 31°C for 60 min and labeled for cell surface VSV-G. (b) The extent of VSV-G transport was measured as indicated in the Materials and methods and is expressed as the ratio of cell surface to total VSV-G fluorescence. The data shown are representative of two experiments with n = 15 for all data points in each experiment.