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. 2003 Feb 3;160(3):375–385. doi: 10.1083/jcb.200208179

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Copyright © 2003, The Rockefeller University Press

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Figure 5. — Inhibition of Cdc42 activity prevents consolidation of a single leading edge upon stimulation by a uniform concentration of chemoattractant. (a and b) Migration of differentiated HL-60 cells in a uniform concentration (100 nM) of fMLP. Cells expressed PH-Akt-GFP alone (a) or in combination with Cdc42N17 (b). (c) Outlines of cells migrating in a uniform concentration (100 nM) of fMLP. Each set of outlines represents a cell observed at 1-min intervals (denoted by colors as indicated), from 2 to 5 min after exposure to fMLP. Small circles in each outline represent the center of a PH-Akt-GFP–containing lamella at the cell periphery. The first control cell (left) and the first Cdc42N17-expressing cell (middle) are the same cells as those depicted in a and b, respectively. In a and b, the top images show the morphology of a single cell by Nomarski microscopy at the indicated times after chemoattractant stimulation; the bottom images show spatial localization of PH-Akt-GFP in the same cell at the same time points. Arrows identify leading edges. Bars, 10 μm. The cells in a and b with the outlines are shown in videos 1 and 2, respectively, available at http://www.jcb.org/cgi/content/full/jcb.200208179/DC1.