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. 2003 Feb 3;160(3):355–364. doi: 10.1083/jcb.200209022

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Copyright © 2003, The Rockefeller University Press

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Figure 6. — Localization of Dyn1–3GFP in living wild-type cells. (A) DIC and movie frames of Dyn1–3GFP fluorescence in a wild-type cell (YJC2772). Each fluorescence image is a two-dimensional projection of a 4-μm Z-stack of confocal images. The time elapsed in seconds is indicated. Dyn1–3GFP is observed as a dot that moves away from and toward the bud (arrows). Dyn1–3GFP sometimes appears as a linear streak (t = 28, 84, and 147 s). Dyn1–3GFP is also observed as stationary cortical dots, but only in the mother (see Videos 10 and 11, available at http://www.jcb.org/cgi/content/full/jcb.200209022/DC1). (B) Dyn1–3GFP colocalizes with the distal ends of cytoplasmic microtubules. DIC, Dyn1–3GFP, and CFP–Tub1 wide-field fluorescence images of wild-type cells (YJC2914) at G1 (top), preanaphase (middle), and anaphase (bottom). The merged images show cytoplasmic Dyn1–3GFP dots (red) at the distal ends of microtubules (blue).