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. 2003 Feb 3;160(3):355–364. doi: 10.1083/jcb.200209022

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Copyright © 2003, The Rockefeller University Press

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Figure 7. — Localization of Dyn1–3GFP in living arp1 Δ , num1 Δ , and pac1 Δ cells. (A) DIC and Dyn1–3GFP fluorescence images of isogenic wild-type and mutant cells. The video camera and microscope settings were the same for the different strains. arp1Δ and num1Δ cells showed increased fluorescence intensity for Dyn1–3GFP dots in the bud. pac1Δ cells showed the absence of cytoplasmic Dyn1–3GFP motile dots. See Videos 12–15 (available at http://www.jcb.org/cgi/content/full/jcb.200209022/DC1). (B) Relative fluorescence intensity of motile Dyn1–3GFP dots in wild-type, arp1Δ, and num1Δ cells. The average corrected fluorescence per dot is plotted; n = 72 dots for wild type, 91 dots for arp1Δ, 84 dots for num1Δ. Error bars represent standard error. Strains: DYN1–3GFP, YJC2772; DYN1–3GFP arp1Δ, YJC2908; DYN1–3GFP num1Δ, YJC2910; DYN1–3GFP pac1Δ, YJC2912.