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. 2003 Feb 3;160(3):341–353. doi: 10.1083/jcb.200211048

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Copyright © 2003, The Rockefeller University Press

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Figure 2. — Rae1 is not essential for nuclear export of mRNA. (A) Overview of the experimental design. Blastocysts from intercrosses of Rae1^+/− mice were cultured for ∼4–5 d and then analyzed by immunostaining or in situ hybridization. (B–C') Double staining of E8.5 embryonic outgrowths with a polyclonal antibody against mouse Rae1(188–368) (Pritchard et al., 1999) and monoclonal antibody mAb414, a marker of the NPC (Wu et al., 2001). Shown are representative high-resolution images of trophectoderm cells. (D and E) Immunostaining of E8.5 embryonic outgrowths with a polyclonal antibody against Nup98(151–224) (Wu et al., 2001). Shown are high-resolution images of trophectoderm cells. (F and G) Localization of poly(A)⁺ RNA in trophectoderm cells from E8.5 Rae1^+/+ and Rae1^−/− outgrowths. A FITC–oligo(dT)₅₀ probe was used for visualization of poly(A)⁺ by in situ hybridization. (H and I) Trophoblast cells stained with a polyclonal antibody against human Tap (Braun et al., 1999).