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. 2003 Aug 4;162(3):383–390. doi: 10.1083/jcb.200301073

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Copyright © 2003, The Rockefeller University Press

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).

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Figure 5. — Furrow abscission and regression in micromanipulated cells. Unless indicated, time is given in minutes. (A) An aster-containing pocket, produced as in Fig. 1, undergoes successful furrow initiation (0–68 min, arrows) and ingression (88), but fails in abscission due to furrow regression (16 h). (B) A cell lacking both asters and pronuclei, manipulated as in Fig. 2, is fully capable of furrow induction (12–45, arrows), ingression (72), and normal abscission (17.1 h; see Video 4, available at http://www.jcb.org/cgi/content/full/jcb.200301073/DC1). (B') Rhodamine dextran microinjected into one daughter cell gradually flows into the other in both visually abscised experimental cells (aster removal, 17.3–17.8 h after cleavage initiation) and nonmanipulated controls (control, 17.2–17.7 h after cleavage initiation). (C) Despite normal furrow induction and ingression (155–243, arrows), cells containing only microtubules, manipulated as in Fig. 3, usually fail to separate (315–390, arrow; see Video 5, available at http://www.jcb.org/cgi/content/full/jcb.200301073/DC1). An exception is shown in Fig. S2, available at http://www.jcb.org/cgi/content/full/jcb.200301073/DC1. Bars, 10 μm.