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. 2003 Aug 4;162(3):425–434. doi: 10.1083/jcb.200302138

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Copyright © 2003, The Rockefeller University Press

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Figure 6. — Budding of Gag ^Δp6 –Hrs ^ΔN is dependent on the P(S/T)AP-binding activity of Tsg101. Top, Western blot showing relative levels of Gag–Hrs^ΔN VLP release. Middle, Western blot showing cytoplasmic expression levels of Gag–Hrs^ΔN. Bottom, Anti-Tsg101 Western blot showing depletion of endogenous Tsg101 protein with siRNA, and reexpression of exogenous, siRNA-resistant Tsg101-Flag proteins (Tsg101*). 293T cells were cotransfected as described in the Materials and methods with the following exceptions: (1) lane 1 received no RNA; and (2) lane 5 was a mock transfection.