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| Construct | NH4 +-ATPase | Actin-activated ATPase
|
Actin velocityb | |
|---|---|---|---|---|
|
|
|
Km
|
Vmax
|
|
| s−1 | μM | s−1 | μms−1 | |
| wt-HMMa | 24.2 ± 2.8 | 20 ± 4 | 2.5 ± 0.2 | 1.6 ± 0.2 (58) |
| E470A-HMMa | 0.01 | ND | ND | 0.00 |
| E470A/wt-HMMa | 12.0 ± 0.8 | 27 ± 7 | 1.8 ± 0.2 | 1.5 ± 0.2 (61) |
| S1-neo | ND | 100 ± 44 | 1.8 ± 0.5 | 0.5 ± 0.1 (17) |
ND, not determined.
Values obtained from Rovner et al. (2003).
Numbers in brackets denote number of filaments sampled.
