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| Construct
|
Step sizea
|
Step durationb
|
|---|---|---|
| nm | ms | |
| wt-HMM | 10.2 ± 0.6 (19) | 104 ± 13 (15) |
| S1-neo | 4.4 ± 0.7 (10) | 189 ± 37 (10) |
| E470A/wt-HMM | 10.4 ± 0.6 (11) | 106 ± 10 (9) |
Numbers in brackets denote number of single molecules sampled. Errors are given as SEM values.
ANOVA test indicates wt-HMM and S1-neo step sizes are statistically different, whereas wt-HMM and E470A/wt-HMM are not.
[ATP] = 10 μm.
