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. 2003 Aug 4;162(3):469–479. doi: 10.1083/jcb.200212067

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Copyright © 2003, The Rockefeller University Press

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Figure 9. — Distribution of PAR-4 and nestin during cell division of ES-J1 ES cells at NP2 stage of differentiation. Multiple, indirect immunofluorescence staining of differentiating ES-J1 cells 48 h on replating of EB8 cells (NP2 stage). PAR-4, (Alexa^® 546, red); nestin, (Alexa^® 488, green); Hoechst (blue). (A) Mitotic cell in anaphase/telophase, overlay of PAR-4, nestin, and Hoechst staining. Note the strict asymmetric distribution of PAR-4 and nestin. (B) Cluster of neuroprogenitor cells, overlay of PAR-4, nestin, and Hoechst staining. Arrowheads indicate two apoptotic PAR-4–positive cells that did not show any expression of nestin.

Figure 9. — Distribution of PAR-4 and nestin during cell division of ES-J1 ES cells at NP2 stage of differentiation. Multiple, indirect immunofluorescence staining of differentiating ES-J1 cells 48 h on replating of EB8 cells (NP2 stage). PAR-4, (Alexa^® 546, red); nestin, (Alexa^® 488, green); Hoechst (blue). (A) Mitotic cell in anaphase/telophase, overlay of PAR-4, nestin, and Hoechst staining. Note the strict asymmetric distribution of PAR-4 and nestin. (B) Cluster of neuroprogenitor cells, overlay of PAR-4, nestin, and Hoechst staining. Arrowheads indicate two apoptotic PAR-4–positive cells that did not show any expression of nestin.