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. 2003 Aug 4;162(3):443–455. doi: 10.1083/jcb.200304008

Figure 7.

Figure 7.

Cellular markers associated with virus-containing vacuoles in MDM. (A) Cryosections of MDM infected with HIV-1 Ba-L were double labeled with rabbit anti-LAMP-2 and PAG10, and virions were identified with anti-p17/PAG15. LAMP-2 was not highly expressed on these MDM, and hence only few gold particles could be observed, but there was some labeling of virus-containing vacuoles (white arrowheads). In addition, a lysosome (L) and membrane tubules are labeled nearby (arrow). (B and C) Cryosections of MDM infected with HIV-1 Ba-L were double labeled with antibodies against CD81 (B) or CD82 (C), whereas virions were identified with anti-p17. Both CD81 and CD82 are associated with the virus-containing vacuoles and can sometimes be found on the virions (black arrowheads). Labeling with anti-CD81 is significantly stronger than with anti-CD82. Bars, 100 nm.