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. 2003 Jul 7;162(1):85–97. doi: 10.1083/jcb.200301022

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Copyright © 2003, The Rockefeller University Press

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Figure 1. — Localization of Rho1p and Fks1p/2p in cells shifted to 37°C. Cells were cultured in YPD at 25°C, shifted to 37°C and cultured for 2 h. Cultured cells were fixed with formaldehyde and then stained for immunofluorescence microscopy with the anti-Rho1p antibody (left) or the anti-Fks1p/2p antibody (right). Strains used were as follows: wild-type (YPH500), sec12, sec1, and sec1 end4.