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. 2003 Jul 7;162(1):47–57. doi: 10.1083/jcb.200303019

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Copyright © 2003, The Rockefeller University Press

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Figure 1. — Cloning the PF2 gene. (A) Partial restriction maps of the region containing the PF2 gene from wild-type and pf2-4 (9B11). Also indicated is the location of the NIT1 plasmid insertion in 9B11, now known as pf2-4, and the position of the flanking clone FC-1 (black box) representing the NotI/BamHI restriction fragment recovered from the 9B11 minilibrary. S, SacI sites; N, NotI sites. (B) Southern blot of wild-type (wt) and pf2-4 genomic DNA digested with the indicated restriction enzymes and hybridized with FC-1. (C) Alignment of three overlapping phage clones recovered with FC-1 (black boxes) and two plasmid clones, derived from λG1, that contain the PF2 gene. Plasmid p9-3HA is the epitope-tagged PF2 construct. Also shown are the number of rescued strains obtained by cotransformation of pf2-4 and pf2-1 with the selected clones. nd, not determined.

Figure 1. — Cloning the PF2 gene. (A) Partial restriction maps of the region containing the PF2 gene from wild-type and pf2-4 (9B11). Also indicated is the location of the NIT1 plasmid insertion in 9B11, now known as pf2-4, and the position of the flanking clone FC-1 (black box) representing the NotI/BamHI restriction fragment recovered from the 9B11 minilibrary. S, SacI sites; N, NotI sites. (B) Southern blot of wild-type (wt) and pf2-4 genomic DNA digested with the indicated restriction enzymes and hybridized with FC-1. (C) Alignment of three overlapping phage clones recovered with FC-1 (black boxes) and two plasmid clones, derived from λG1, that contain the PF2 gene. Plasmid p9-3HA is the epitope-tagged PF2 construct. Also shown are the number of rescued strains obtained by cotransformation of pf2-4 and pf2-1 with the selected clones. nd, not determined.