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. 2003 Jul 7;162(1):47–57. doi: 10.1083/jcb.200303019

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Copyright © 2003, The Rockefeller University Press

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Figure 5. — Localization of the epitope-tagged PF2 protein in Chlamydomonas flagella. The epitope-tagged, rescued strain, pf2-4r:3HA, was stained with an antibody against the HA tag and then imaged by differential interference contrast (A and C) or indirect immunofluorescence light microscopy (B and D). The HA-tagged PF2 is present along the entire length of the flagella in pf2-4r:3HA cells and can also been seen in two spots in the basal body region (B and D). pf2-4 mutant (E and F) or wild-type cells (G and H) labeled with the HA antibody or secondary antibody alone (not depicted) show only background cell body autofluorescence.