Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2003 Jul 7;162(1):71–84. doi: 10.1083/jcb.200302169

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2003, The Rockefeller University Press

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).

PMC Copyright notice

Figure 2. — p97 binding to cofactors and membrane requires its N domain. (A) p97 binding to cofactors. Purified His-tagged p97 proteins (His-p97) were added to rat liver cytosol in the presence of 1 M NaCl. After dilution of the salt to 150 mM, p97 was immunoprecipitated with His antibodies. Bound proteins were detected by immunoblotting with antibodies to the indicated proteins. (B) Association of p97 with the membrane. Purified His-p97 proteins were added to permeabilized astrocytoma cells. After incubation, the cells were fractionated into membrane (P) and cytosol (S) fractions. The amount of His-p97 in each fraction was determined by immunoblotting with His antibodies (top). Immunoblotting for calnexin served as a loading control for the membrane fraction (bottom).

Figure 2. — p97 binding to cofactors and membrane requires its N domain. (A) p97 binding to cofactors. Purified His-tagged p97 proteins (His-p97) were added to rat liver cytosol in the presence of 1 M NaCl. After dilution of the salt to 150 mM, p97 was immunoprecipitated with His antibodies. Bound proteins were detected by immunoblotting with antibodies to the indicated proteins. (B) Association of p97 with the membrane. Purified His-p97 proteins were added to permeabilized astrocytoma cells. After incubation, the cells were fractionated into membrane (P) and cytosol (S) fractions. The amount of His-p97 in each fraction was determined by immunoblotting with His antibodies (top). Immunoblotting for calnexin served as a loading control for the membrane fraction (bottom).