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. 2003 Jul 7;162(1):59–69. doi: 10.1083/jcb.200302084

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Copyright © 2003, The Rockefeller University Press

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Figure 4. — Generation of mitochondrial- and the ER- targeted Bak mutants. (A) Schemes of the Bak mutants. The carboxy-terminal transmembrane domain of Bak was deleted (ΔC) or replaced with the mitochondria-targeting sequence of ActA (ActA) or the ER-targeting sequence of cb5. (B) Targeting the Bak mutants to mitochondria and the ER. Bak mutants were expressed in MCF7 cells. Mitochondria marker COX IV and the ER marker calnexin were visualized by immunofluorescence. Note that the Bak-ActA mutant colocalized with COX IV but not calnexin, and the Bak-cb5 mutant colocalized with calnexin but not COX IV.