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. 2003 Jul 7;162(1):59–69. doi: 10.1083/jcb.200302084

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Copyright © 2003, The Rockefeller University Press

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Figure 7. — ER-targeted Bak can induce selective cleavage of caspase 12, but not of caspase 7. bax ⁻ ^/ ⁻ bak ⁻ ^/ ⁻ cells were infected with GFP vector control, Bak-IRES-GFP, Bak-ActA-IRES-GFP, Bak-cb5-IRES-GFP, and Bak-ΔC-IRES-GFP at a high multiplicity. After infection, cells were lysed. Caspase 12, caspase 7, or PARP were detected by immunoblotting using respective antibodies.