Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2003 Jul 7;162(1):23–35. doi: 10.1083/jcb.200303098

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2003, The Rockefeller University Press

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).

PMC Copyright notice

Figure 5. — Normal chromosome morphology over insert regions by light microscopy. In native chromosomes, the vector insert appears as a band going over the entire width of the chromosome, or as a pair of spots within normal diameter chromosome. (A) Con-1 clone showing insert across entire chromosome width. (B) Con-610 clone with single insert site. (C) dSAR-d11 clone showing two insert sites. (D) dSAR-g12 clone showing two insert sites. (column 1) Lac repressor immunostaining (green) and DAPI staining (red) of metaphase cells; (columns 2–4) metaphase chromosomes at vector integration sites: lac repressor immunostaining (green), DAPI staining (red, or grayscale). (column 5) Chromosome structure at the regions of vector insertion for cells expressing GFP–lacI–NLS fusion protein. Bars, 1 μm (white, column 1; black, columns 2–5).