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. 2003 Jan 6;160(1):53–64. doi: 10.1083/jcb.200210084

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Copyright © 2003, The Rockefeller University Press

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Figure 6. — MOM targeting also requires two basic residues downstream of the TM of Bcl-x and Bcl-2 accumulates on mitochondria by increasing the basicity surrounding its TM. (A and B) Anti–Bcl-2 and anti–Bcl-x immunofluorescence analysis of HeLa or R6 cells (as indicated) transiently transfected with FLAG-tagged Bcl-x_L mutants that had their COOH-terminal basic residues exchanged to serines (A) or FLAG-tagged Bcl-2 mutants that contained additional basic residues at their COOH termini (B). In addition, the localization of COOH-terminal FLAG-tagged Bcl-x_L is shown (Bcl-x_L–FLAG). The mitochondrial colocalization markers are cytochrome c or TOM20 as indicated. (C) Anti-FLAG Western blots of subcellular fractions from HEK293 cells expressing the mutants described under A and B.

Figure 6. — MOM targeting also requires two basic residues downstream of the TM of Bcl-x and Bcl-2 accumulates on mitochondria by increasing the basicity surrounding its TM. (A and B) Anti–Bcl-2 and anti–Bcl-x immunofluorescence analysis of HeLa or R6 cells (as indicated) transiently transfected with FLAG-tagged Bcl-x_L mutants that had their COOH-terminal basic residues exchanged to serines (A) or FLAG-tagged Bcl-2 mutants that contained additional basic residues at their COOH termini (B). In addition, the localization of COOH-terminal FLAG-tagged Bcl-x_L is shown (Bcl-x_L–FLAG). The mitochondrial colocalization markers are cytochrome c or TOM20 as indicated. (C) Anti-FLAG Western blots of subcellular fractions from HEK293 cells expressing the mutants described under A and B.