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. 2003 Jan 6;160(1):101–112. doi: 10.1083/jcb.200209127

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Copyright © 2003, The Rockefeller University Press

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Figure 2. — CFP/YFP micro-FRET microscopy imaging of AKAP79 binding to CaN and PKA in living cells. (A) Plasma membrane/cortical colocalization (CFP/YFP Overlay) and direct binding (FRET^C) seen for CaNA–YFP (green) and AKAP79–CFP WT but not ΔCaN (Δ315–360) (blue) in live COS7 cells. (B) Plasma membrane/cortical colocalization (CFP/YFP Overlay) and direct binding (FRET^C) seen for PKA-RIIα–YFP (green) and AKAP79–CFP WT but not ΔPKA (1–361) (blue) in live COS7 cells. (C) Negative control showing no FRET^C for AKAP79–CFP (blue) and AKAP79–YFP (green) that colocalize at the plasma membrane (CFP/YFP Overlay) but do not bind to each other. (D) Negative control showing no FRET^C for AKAP79–CFP (blue) and AKAP18(1–16)–YFP (green) that colocalize (CFP/YFP Overlay) but do not bind to each other. Bars, ∼15 μm.